Vang-like Protein 2/VANGL2 Antibody

Western Blot: Vang-like Protein 2/VANGL2 Antibody [NB100-59736] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Bv, Ze
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Vang-like Protein 2/VANGL2 Antibody Summary

• Description: Novus Biologicals Goat Vang-like Protein 2/VANGL2 Antibody (NB100-59736) is a polyclonal antibody validated for use in WB and ELISA. Anti-Vang-like Protein 2/VANGL2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QKYLRTTKQQPYH corresponding to internal region according to NP_065068.1.
• Epitope: QKYLRTTKQQPYH
• Predicted Species: Human (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VANGL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx 55 kDa band observed in mouse brain lysates (calculated MW of 59.7kDa according to NP_065068.1).
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Vang-like Protein 2/VANGL2 Antibody

• KIAA1215Loop-tail protein 1 homolog
• loop-tail-associated protein
• LPP1
• LTAP
• LTAPVan Gogh-like protein 2
• MGC119403
• MGC119404
• STB1
• STB1Strabismus 1
• STBM
• STBM1
• Strabismus
• vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (Drosophila)
• Vang like Protein 2
• VANGL2
• vang-like 2 (van gogh, Drosophila)
• Vang-like Protein 2

Background

VANGL2 is involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. It plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. The VANGL2 gene encodes a homolog of Drosophila 'strabismus/Van Gogh' (Stbm/Vang), a component of the frizzled-dishevelled tissue polarity pathway.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vang-like Protein 2/VANGL2 Antibody (NB100-59736)(1)

Publication using NB100-59736

• Kibar Z, Vogan KJ, Groulx N et al. Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail. Nat Genet 2001-07-01 [PMID: 11431695]

Bioinformatics

• Gene Symbol: VANGL2
• Entrez:
  • 57216(Human)
  • 93840(Mouse)
• Uniprot:
  • Q9ULK5()