VAM1 Antibody

Images

 
Western Blot: VAM1 Antibody [NB100-1366] - Western blot analysis of PALS2 / VAM1 / MPP6 in 293 lysate using NB100-1366 at 0.5 ug/ ml.
Western Blot: VAM1 Antibody [NB100-1366] - (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VAM1 Antibody Summary

Immunogen
Peptide with sequence QQVLENLTELPSS-C corresponding to N-Terminus according to NP_057531.2.
Localization
Plasma membrane
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPP6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 60 kDa band observed in HEK293 lysates (calculated MW of 61.1 kDa band according to NP_057531.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control
293 Lysate (NB800-PC6)
293 Lysate (NB800-PC6)
VAM1 Lysate (NBL1-13205)
Brain Lysate (NB820-59177)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-1366.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAM1 Antibody

  • MAGUK protein p55T
  • membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
  • p55T
  • protein associated with Lin7 2
  • VAM-1PALS2VAM1MAGUK p55 subfamily member 6
  • Veli-associated MAGUK 1

Background

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like, ZO1-like, p55-like and LIN2-like, based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAM1 Antibody (NB100-1366)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MPP6
Entrez
Uniprot