VAM1 Antibody

Western Blot: VAM1 Antibody [NB100-1366] - Staining of HEK293 cell lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by Western blot using chemiluminescence.

Immunohistochemistry-Paraffin: VAM1 Antibody [NB100-1366] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VAM1 Antibody Summary

• Description: Novus Biologicals Goat VAM1 Antibody (NB100-1366) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-VAM1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence QQVLENLTELPSS-C corresponding to N-Terminus according to NP_057531.2.
• Localization: Plasma membrane
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MPP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 60 kDa band observed in HEK293 lysates (calculated MW of 61.1 kDa band according to NP_057531.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  293 Whole Cell Lysate
  293 Whole Cell Lysate
  VAM1 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VAM1 Antibody

• MAGUK protein p55T
• membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
• p55T
• protein associated with Lin7 2
• VAM-1PALS2VAM1MAGUK p55 subfamily member 6
• Veli-associated MAGUK 1

Background

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like, ZO1-like, p55-like and LIN2-like, based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAM1 Antibody (NB100-1366)(1)

Publication using NB100-1366

• Kamberov E, Makarova O, Roh M et al. Molecular cloning and characterization of Pals, proteins associated with mLin-7. J Biol Chem 2000-04-14 [PMID: 10753959]

Bioinformatics

• Gene Symbol: MPP6
• Entrez:
  • 51678(Human)
  • 56524(Mouse)
• Uniprot:
  • NP_057531.(Human)