VAM1 Antibody

Images

 
Western Blot: VAM1 Antibody [NB100-1366] - Staining of HEK293 cell lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by Western blot using chemiluminescence.
Immunohistochemistry-Paraffin: VAM1 Antibody [NB100-1366] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VAM1 Antibody Summary

Immunogen
Peptide with sequence QQVLENLTELPSS-C corresponding to N-Terminus according to NP_057531.2.
Localization
Plasma membrane
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPP6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 60 kDa band observed in HEK293 lysates (calculated MW of 61.1 kDa band according to NP_057531.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
293 Whole Cell Lysate
293 Whole Cell Lysate
VAM1 Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
VAM1 Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-1366.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAM1 Antibody

  • MAGUK protein p55T
  • membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
  • p55T
  • protein associated with Lin7 2
  • VAM-1PALS2VAM1MAGUK p55 subfamily member 6
  • Veli-associated MAGUK 1

Background

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like, ZO1-like, p55-like and LIN2-like, based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAM1 Antibody (NB100-1366)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol MPP6
Entrez
Uniprot