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VAM1 Overexpression Lysate

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Western Blot: VAM1 Overexpression Lysate (Adult Normal) [NBL1-13205] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VAM1.
Western Blot: VAM1 Overexpression Lysate (Adult Normal) [NBL1-13205] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VAM1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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VAM1 Overexpression Lysate Summary

Description
Transient overexpression lysate of membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) (MPP6) The lysate was created in HEK293T cells, using Plasmid ID RC205667 and based on accession number NM_016447. The protein contains a C-MYC/DDK Tag.
Gene
MPP6

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
60.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VAM1 Overexpression Lysate

  • MAGUK protein p55T
  • membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
  • p55T
  • protein associated with Lin7 2
  • VAM-1PALS2VAM1MAGUK p55 subfamily member 6
  • Veli-associated MAGUK 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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NB300-186
Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.
Immunocytochemistry/Immunofluorescence: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - SynCAM/CADM1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
(1 Publication)
Species: Hu, Rt
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Guanylate kinase
Antibody (OTI4A8)
NBP2-00594
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Hela cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Jurkat cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Guanylate kinase antibody, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
PALS1/MPP5 Antibody
NB300-952
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: PALS1/MPP5 Antibody [NB300-952] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
LIN7 Antibody
NBP2-99481
Immunocytochemistry/Immunofluorescence: LIN7 Antibody [NBP2-99481] - Immunofluorescence staining of LIN7 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LIN7 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.
Western Blot: LIN7 Antibody [NBP2-99481] - Lane A: Mouse brain tissue lysateLane B: Rat brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa
Immunoprecipitation: LIN7 Antibody [NBP2-99481] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :45 kDa
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Exosome component
10 Antibody
NBP1-32870
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
(2 Reviews)
(3 Publications)
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
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(1E11-1G11)
H00004354-M01
Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.
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MPP3 Antibody
NBP1-85014
Immunocytochemistry/Immunofluorescence: MPP3 Antibody [NBP1-85014] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
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SAP102 Antibody
NBP1-87691
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Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.
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IGSF4B/SynCAM3/CADM3 A ...
AF3678
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(1 Publication)
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MPP2 Antibody
NBP3-17060
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NB600-1229
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(1 Publication)
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PSD93 Antibody
(S18-30)
NBP2-12912
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Western Blot: PSD93 Antibody (S18-30) [NBP2-12912] - analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
LIN7C Antibody
NBP2-14193
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: IHC, IHC-P
CASK Antibody -
BSA Free
NBP2-41181
Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.
Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, Simple Western, WB
MUPP1 Antibody
NBP1-87839
Immunohistochemistry-Paraffin: MUPP1 Antibody [NBP1-87839] - Staining of human stomach shows strong membranous positivity in glandular cells.
(1 Review)
Species: Hu
Applications: IHC, IHC-P
DR3/TNFRSF25
Antibody (59204) [Unc ...
MAB943
Mouse splenocytes either (A) untreated or (B) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Western blot shows lysates of human CD8+ T cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse DR3/TNFRSF25 Monoclonal Antibody (Catalog # MAB943) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DR3/TNFRSF25 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
Dermatopontin
Antibody
AF4629
Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human Dermatopontin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4629) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dermatopontin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
LIN10 Antibody
NBP1-88454
Immunohistochemistry-Paraffin: LIN10 Antibody [NBP1-88454] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: LIN10 Antibody [NBP1-88454] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: LIN10 Antibody [NBP1-88454] - Staining of human cell line A-431 shows localization to nuclear speckles & cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DREF Antibody
(OTI5A2) - Azide and ...
NBP2-71419
Western Blot: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DREF.
Flow Cytometry: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by DREF1 antibody.
Immunohistochemistry: DREF Antibody (OTI5A2) - Azide and BSA Free [NBP2-71419] - Analysis of Adenocarcinoma of Human colon tissue.
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
VAM1
Overexpression Lysate
NBL1-13205
VAM1 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol MPP6