UVSSA Antibody - BSA Free

Western Blot: UVSSA Antibody [NBP1-32598] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: UVSSA Antibody [NBP1-32598] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UVSSA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UVSSA Antibody - BSA Free (NBP1-32598) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-UVSSA Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human UVSSA. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UVSSA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UVSSA Antibody - BSA Free

• hypothetical protein LOC57654
• KIAA1530
• MGC117169

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for UVSSA Antibody (NBP1-32598)(7)

Publications using NBP1-32598

• Y van der We, H Golan-Berm, TET Mevissen, K Apelt, R González-P, J Goedhart, EE Heilbrun, ACO Vertegaal, D van den He, JC Walter, S Adar, MS Luijsterbu The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II Nat Commun, 2020-04-30;11(1):2104. 2020-04-30 [PMID: 32355176]
• Schwertman P, Lagarou A, Dekkers DH et al. UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Nat Genet 2012-05-01 [PMID: 22466611] (WB, Human)
• Nakazawa Y, Sasaki K, Mitsutake N et al. Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair. Nat Genet. 2012-04-01 [PMID: 22466610] (Human)
• van der Weegen Y, Berman HG, Mevissen TET, Apelt K The sequential and cooperative action of CSB, CSA and UVSSA targets the TFIIH complex to DNA damage-stalled RNA polymerase II bioRxiv (WB, Human)
• Sato M, Liebau AW, Liu Z, et al. The UVSSA complex alleviates MYC-driven transcription stress The Journal of cell biology 2021-02-01 [PMID: 33404608]
• Weegen Y Unravelling molecular mechanisms in transcription-coupled nucleotide excision repair Thesis 2022-01-01
• van der Weegen Y, Berman HG, Mevissen TET, Apelt K The sequential and cooperative action of CSB, CSA and UVSSA targets the TFIIH complex to DNA damage-stalled RNA polymerase II Nat Commun 2020-11-07 [PMID: 33159085]

Bioinformatics

• Gene Symbol: UVSSA
• Entrez:
  • 57654(Human)
• Uniprot:
  • Q2YD98(Human)