USP52 Antibody (6A7)

Immunocytochemistry/Immunofluorescence: USP52 Antibody (6A7) [H00009924-M01] - Analysis of monoclonal antibody to PAN2 on HeLa cell . Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 6A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

USP52 Antibody (6A7) Summary

• Immunogen: USP52 (NP_055686, 1099 a.a. ~ 1198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVLAL
• Localization: Cytoplasmic and Nuclear. Note=Shuttles between nucleus and cytoplasm.
• Specificity: USP52 - ubiquitin specific peptidase 52
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP52 Antibody (6A7)

• EC 3.1.13.4
• FLJ39360
• hPan2
• Inactive ubiquitin carboxyl-terminal hydrolase 52
• KIAA0710PAB-dependent poly(A)-specific ribonuclease subunit 2
• NALP4
• PABP-dependent poly(A) nuclease 2
• PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• PAN2 polyA specific ribonuclease subunit
• PYPAF4
• ubiquitin specific peptidase 52
• ubiquitin specific protease 52
• USP52

Background

USP52 shows homology to the ubiquitin specific peptidase family. However, it has no ubiquitin carboxyl terminal hydrolase activity since it lacks a conserved catalytic Cys at position 525. It functions in cytoplasmic mRNA decay as part of the Pan nuclease complex which shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAN2
• Entrez:
  • 9924(Human)
• Uniprot:
  • NP_055686(Human)