USP47 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP47 Antibody [NBP1-85942] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: USP47 Antibody [NBP1-85942] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: USP47 Antibody [NBP1-85942] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Analysis in human cell line K562.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

USP47 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP47 Antibody - BSA Free (NBP1-85942) is a polyclonal antibody validated for use in IHC and WB. Anti-USP47 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGK
• Predicted Species: Mouse (93%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP47
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  USP47 Protein (NBP1-85942PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for USP47 Antibody - BSA Free

• Deubiquitinating enzyme 47
• DKFZp686C13257
• EC 3.4.19.12
• FLJ20727
• Trf (TATA binding protein-related factor)-proximal homolog (Drosophila)
• Trf (TATA binding protein-related factor)-proximal homolog
• TRFP
• ubiquitin carboxyl-terminal hydrolase 47
• ubiquitin specific peptidase 47
• ubiquitin specific protease 47
• ubiquitin thioesterase 47
• Ubiquitin thiolesterase 47
• Ubiquitin-specific-processing protease 47
• USP47

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP47 Antibody (NBP1-85942)(1)

Publication using NBP1-85942

• Xiao Q, Tang Y, Xia J et al. Ubiquitin-specific protease 47 is associated with vascular calcification in chronic kidney disease by regulating osteogenic transdifferentiation of vascular smooth muscle cells Renal Failure 2022-12-31 [PMID: 35509185]

Bioinformatics

• Gene Symbol: USP47