USP47 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP47 Antibody [NB100-57487] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP47 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: USP47 Antibody [NB100-57487] - Detection of Human USP47 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody NB100-57487 used for IP at 3 mcg/mg lysate. USP47 was also immunoprecipitated by rabbit anti- USP47 antibody NB100-57486, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Ch
• Applications: IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP47 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP47 Antibody - BSA Free (NB100-57487) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031).
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP47
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg
• Application Notes: This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP47 Antibody - BSA Free

• Deubiquitinating enzyme 47
• DKFZp686C13257
• EC 3.4.19.12
• FLJ20727
• Trf (TATA binding protein-related factor)-proximal homolog (Drosophila)
• Trf (TATA binding protein-related factor)-proximal homolog
• TRFP
• ubiquitin carboxyl-terminal hydrolase 47
• ubiquitin specific peptidase 47
• ubiquitin specific protease 47
• ubiquitin thioesterase 47
• Ubiquitin thiolesterase 47
• Ubiquitin-specific-processing protease 47
• USP47

Background

USP47 belongs to the peptidase C19 family and may be catalytically inactive although it seems to have kept all necessary active site residues. It is expressed in skeletal muscle, heart and testis and there are 3 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP47
• Entrez:
  • 55031(Human)
• Uniprot:
  • NP_060414.3(Human)