USP37 Antibody

Western Blot: USP37 Antibody [NB110-40709] - Detection of Human USP37 on HeLa whole cell lysate using NB110-40709. USP37 was also immunoprecipitated by rabbit anti-USP37 antibody NB110-40696.

Immunohistochemistry: USP37 Antibody [NB110-40709] - Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- USP37 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Overexpression of USP37 leads to enhanced survival in response to replication stress while its depletion leads to reduced survival in osteosarcoma cells with increased accumulation of USP37 in cytoplasm. A The clonogenic potential was examined by Colony formation assay. U2OS osteosarcoma cells were transfected with Myc USP37 for overexpressing USP37 while SiRNA was used to reduce expression of USP37, following which cells were treated with different concentrationsn of HU (Hydroxy Urea) for 24 h., then washed and fresh media was added. Subsequently colonies were allowed to develop for 7 days. B Osteosarcoma cell U20S cells were treated with 300 uM HU for 24 h and processed for IF to see USP37 expression. C Osteosarcoma cell line MG-63 was treated with 300 uM HU for 24 h. and processed for IF to see USP37 expression. D Western blotting to see the expression of USP37 and corresponding DNA damage marker ϒH2AX after induction of replication stress in U2OS whole cell lysate. E Nuclear and cytoplasmic fractionation was carried out on U2OS cells after treatment by an escalating dose of HU and western blotting was done to assess the level of USP37 in the nuclear and cytoplasmic fraction Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37118828), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Overexpression of USP37 leads to enhanced survival in response to replication stress while its depletion leads to reduced survival in osteosarcoma cells with increased accumulation of USP37 in cytoplasm. A The clonogenic potential was examined by Colony formation assay. U2OS osteosarcoma cells were transfected with Myc USP37 for overexpressing USP37 while SiRNA was used to reduce expression of USP37, following which cells were treated with different concentrationsn of HU (Hydroxy Urea) for 24 h., then washed and fresh media was added. Subsequently colonies were allowed to develop for 7 days. B Osteosarcoma cell U20S cells were treated with 300 uM HU for 24 h and processed for IF to see USP37 expression. C Osteosarcoma cell line MG-63 was treated with 300 uM HU for 24 h. and processed for IF to see USP37 expression. D Western blotting to see the expression of USP37 and corresponding DNA damage marker ϒH2AX after induction of replication stress in U2OS whole cell lysate. E Nuclear and cytoplasmic fractionation was carried out on U2OS cells after treatment by an escalating dose of HU and western blotting was done to assess the level of USP37 in the nuclear and cytoplasmic fraction Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37118828), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

USP37 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 930 and the C-terminus (residue 979) of human Ubiquitin Specific Peptidase 37 using the numbering given in entry NP_065986.1 (GeneID 57695).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP37
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  USP37 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP37 Antibody

• Deubiquitinating enzyme 37
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1594tmp_locus_50
• MGC117261
• ubiquitin carboxyl-terminal hydrolase 37
• ubiquitin specific peptidase 37
• ubiquitin specific protease 37
• ubiquitin thioesterase 37
• Ubiquitin thiolesterase 37
• Ubiquitin-specific-processing protease 37
• USP37

Background

UPS37 is a member of the C19 peptidase family of ubiquitin dependent peptidase proteins. It along with similar family members is believed to participate in the cells ubiquitin-dependent protein catabolic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP37 Antibody (NB110-40709)(2)

Publications using NB110-40709

• Chauhan R, Gupta A, Malhotra L et al. Ubiquitin specific peptidase 37 and PCNA interaction promotes osteosarcoma pathogenesis by modulating replication fork progression Journal of translational medicine 2023-04-28 [PMID: 37118828] (IP, Human)
• Das CM, Taylor P, Gireud M et al. The deubiquitylase USP37 links REST to the control of p27 stability and cell proliferation. Oncogene 2012-06-01 [PMID: 22665064]

Bioinformatics

• Gene Symbol: USP37
• Entrez:
  • 57695(Human)
• Uniprot:
  • Q86T82(Human)