USP34 Antibody

Western Blot: USP34 Antibody [NB100-2863] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-2863 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

Immunohistochemistry-Paraffin: USP34 Antibody [NB100-2863] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USP34 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

USP34 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 3350 and the C-terminus (residue 3395) of human Ubiquitin Specific Protease 34 using the numbering given in entry CAE51938.1 (GeneID 9736).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP34
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP34 Antibody

• Deubiquitinating enzyme 34
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ43910
• KIAA0570
• KIAA0570MGC104459
• KIAA0729
• KIAA0729ubiquitin carboxyl-terminal hydrolase 34
• ubiquitin specific peptidase 34
• ubiquitin specific protease 34
• ubiquitin thioesterase 34
• Ubiquitin thiolesterase 34
• Ubiquitin-specific-processing protease 34
• USP34

Background

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP34 Antibody (NB100-2863)(2)

Publications using NB100-2863

• Wang H, Ju D, Kho DH et al. The ubiquitin specific protease USP34 protects the ubiquitin ligase gp78 from proteasomal degradation Biochem. Biophys. Res. Commun. 2018-12-22 [PMID: 30585151] (WB, Human)
• Lui TT, Lacroix C, Ahmed SM et al. The ubiquitin-specific protease USP34 regulates axin stability and Wnt/beta-catenin signaling. Mol Cell Biol 2011-05-01 [PMID: 21383061]

Bioinformatics

• Gene Symbol: USP34
• Entrez:
  • 9736(Human)
  • 17847(Mouse)
• Uniprot:
  • CAE51938.1(Human)