USP28 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human heart muscle, kidney, liver and skeletal muscle using Anti-TNNT2 antibody (A) NBP1-82905 shows similar protein distribution across tissues to independent antibody NBP1-82904 (B).

Western Blot: USP28 Antibody [NBP1-82905] - Analysis using Anti-USP28 antibody NBP1-82905 (A) shows similar pattern to independent antibody NBP1-82904 (B).

Immunocytochemistry/Immunofluorescence: USP28 Antibody [NBP1-82905] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining shown in green.

Western Blot: USP28 Antibody [NBP1-82905] - Analysis in human cell line A-549.

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human heart muscle shows strong nuclear positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human rectum shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

USP28 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPND
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  USP28 Protein (NBP1-82905PEP)

Reactivity Notes

Mouse, Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for USP28 Antibody - BSA Free

• Deubiquitinating enzyme 28
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1515ubiquitin carboxyl-terminal hydrolase 28
• ubiquitin specific peptidase 28
• ubiquitin specific protease 28
• ubiquitin thioesterase 28
• Ubiquitin thiolesterase 28
• Ubiquitin-specific-processing protease 28
• USP28

Background

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP28 Antibody (NBP1-82905)(1)

Publication using NBP1-82905

• Stadler C, Hjelmare M, Neumann B et al. Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics 2012-04-03 [PMID: 22361696]

Bioinformatics

• Gene Symbol: USP28