USP28 Antibody

Western Blot: USP28 Antibody [NB110-40543] - Whole cell lysate (5 ug) from U2OS, K-562, HEK293T, GaMG, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP28 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: USP28 Antibody [NB110-40543] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-USP28 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: USP28 Antibody [NB110-40543] - Detection of Human USP28 on HeLa whole cell lysate usingy NB110-40543. USP28 was also immunoprecipitated by rabbit anti-USP28 antibodies NB110-40544 and another USP28 Ab.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

USP28 Antibody Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-322
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: 0. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-322

Alternate Names for USP28 Antibody

• Deubiquitinating enzyme 28
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1515ubiquitin carboxyl-terminal hydrolase 28
• ubiquitin specific peptidase 28
• ubiquitin specific protease 28
• ubiquitin thioesterase 28
• Ubiquitin thiolesterase 28
• Ubiquitin-specific-processing protease 28
• USP28

Background

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP28 Antibody (NB110-40543)(1)

Publication using NB110-40543

• Schleicher EM, Dhoonmoon A, Jackson LM et al. The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells Oncogenesis 2022-06-18 [PMID: 35717336] (WB, Human)

Bioinformatics

• Gene Symbol: USP28
• Entrez:
  • 57646(Human)
  • 235323(Mouse)
• Uniprot:
  • Q96RU2()