USP28 Antibody

Images

 
Western Blot: USP28 Antibody [NB110-40543] - Detection of Human USP28 on HeLa whole cell lysate usingy NB110-40543. USP28 was also immunoprecipitated by rabbit anti-USP28 antibodies NB110-40544 and another USP28 Ab.
Immunohistochemistry: USP28 Antibody [NB110-40543] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP28 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: USP28 Antibody [NB110-40543] - FFPE section of human colon adenocarcinoma. Affinity purified rabbit anti-USP28 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: USP28 Antibody [NB110-40543] - FFPE section of human stomach adenocarcinoma. Affinity purified rabbit anti-USP28 used at a dilution of 1:250.
Proximity Ligation Assay: USP28 Antibody [NB110-40543] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human USP28 (NB110-40543). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

USP28 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-322

Alternate Names for USP28 Antibody

  • Deubiquitinating enzyme 28
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1515ubiquitin carboxyl-terminal hydrolase 28
  • ubiquitin specific peptidase 28
  • ubiquitin specific protease 28
  • ubiquitin thioesterase 28
  • Ubiquitin thiolesterase 28
  • Ubiquitin-specific-processing protease 28
  • USP28

Background

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP28
Entrez
Uniprot