USP24 Antibody

Images

 
Western Blot: USP24 Antibody [NB100-40830] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP24 antibody used for WB ...read more
Independent Antibodies: Immunoprecipitation: USP24 Antibody [NB100-40830] - Detection of human USP24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

USP24 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2410 and the C-terminus (residue 2460) of human Ubiquitin Specific Peptidase 24 using the numbering given in entry XP_371254.5 (GeneID 23358).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP24
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
Theoretical MW
294 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
USP24 Knockout 293T Cell Lysate
Publications
Read Publication using NB100-40830.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP24 Antibody

  • Deubiquitinating enzyme 24
  • EC 3.4.19.12
  • FLJ31309
  • KIAA1057ubiquitin carboxyl-terminal hydrolase 24
  • ubiquitin specific peptidase 24
  • ubiquitin specific protease 24
  • ubiquitin thioesterase 24
  • Ubiquitin thiolesterase 24
  • ubiquitin-specific processing protease 24
  • Ubiquitin-specific-processing protease 24
  • USP24

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP24 Antibody (NB100-40830)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for USP24 Antibody (NB100-40830) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-40830AF350
Alexa Fluor 405 NB100-40830AF405
Alexa Fluor 488 NB100-40830AF488
Alexa Fluor 532 NB100-40830AF532
Alexa Fluor 594 NB100-40830AF594
Alexa Fluor 647 NB100-40830AF647
Alexa Fluor 700 NB100-40830AF700
Alexa Fluor 750 NB100-40830AF750
Biotin NB100-40830B
DyLight 350 NB100-40830UV
DyLight 405 NB100-40830V
DyLight 488 NB100-40830G
DyLight 550 NB100-40830R
DyLight 594 NB100-40830DL594
DyLight 650 NB100-40830C
DyLight 680 NB100-40830FR
DyLight 755 NB100-40830IR
HRP NB100-40830H

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Contact Information

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Bioinformatics

Gene Symbol USP24
Entrez
Uniprot