USP22 Antibody

Western Blot: USP22 Antibody [NBP2-20829] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: USP22 Antibody [NBP2-20829] - Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 ug of USP22 antibody [N3C3]. Western blot analysis was performed using USP22 antibody [N3C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

USP22 Antibody Summary

• Description: Novus Biologicals Rabbit USP22 Antibody (NBP2-20829) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human USP22. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Xenopus (97%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP22
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for USP22 Antibody

• Deubiquitinating enzyme 22
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1063ubiquitin carboxyl-terminal hydrolase 22
• KIAA1064
• ubiquitin specific peptidase 22
• ubiquitin specific peptidase 3-like
• ubiquitin specific protease 22
• Ubiquitin Thioesterase 22
• Ubiquitin thiolesterase 22
• ubiquitin-specific processing protease 22
• Ubiquitin-specific-processing protease 22
• USP22
• USP3L

Background

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: USP22
• Uniprot:
  • Q9UPT9()