USP2 Antibody

Immunohistochemistry-Paraffin: USP2 Antibody [NB110-83975] - Analysis of anti-USP2 antibody with human testis at concentration 5 ug/ml.

Product Discontinued

USP2 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from internal region of human USP2.
• Epitope: internal domain of human protein
• Specificity: Human USP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Porcine (94%), Rabbit (94%), Equine (94%), Canine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1.25-2.5 ug/ml
• Application Notes: IHC: NB110-83975 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (89%), Mouse (89%), Rat (89%), Bovine (89%), Hamster (89%), Opossum (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP2 Antibody

• Deubiquitinating enzyme 2
• EC 3.1.2.15
• EC 3.4.19.12,41 kDa ubiquitin-specific protease
• ubiquitin specific peptidase 2
• ubiquitin specific protease 12
• ubiquitin specific protease 2
• ubiquitin specific protease 9
• ubiquitin thioesterase 2
• Ubiquitin thiolesterase 2
• Ubiquitin-specific-processing protease 2
• UBP41
• UBP41ubiquitin carboxyl-terminal hydrolase 2
• USP2
• USP9

Background

UBP, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP2
• Entrez:
  • 9099(Human)
  • 53376(Mouse)
  • 115771(Rat)