USP2 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

USP2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant mouse USP2 isoform 2
  Lys47-Met396
  Accession # NP_932759
• Specificity: Detects mouse USP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human USP2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for USP2 Antibody [Alexa Fluor® 350]

• Deubiquitinating enzyme 2
• EC 3.1.2.15
• EC 3.4.19.12,41 kDa ubiquitin-specific protease
• ubiquitin specific peptidase 2
• ubiquitin specific protease 12
• ubiquitin specific protease 2
• ubiquitin specific protease 9
• ubiquitin thioesterase 2
• Ubiquitin thiolesterase 2
• Ubiquitin-specific-processing protease 2
• UBP41
• UBP41ubiquitin carboxyl-terminal hydrolase 2
• USP2
• USP9

Background

USP2 (Ubiquitin-specific-processing protease 2; also Ubiquitin carboxy-terminal hydrolase 2) is a cytosolic member of the peptidase C19 family of enzymes. It is widely expressed and serves to deubiquitinate select target substrates. USP2 effects are determined by its targets. In cells where it increases Mdm2, p53 is degraded and apoptosis is blocked. In cells where it increases Itch concentration, Flip activity is interrupted and apoptosis is promoted. USP2 is reported to form homo‑oligomers. There are at least two isoform variants. Each is approximately 70 kDa in size, with one showing a 266 aa, and another a 272 aa substitution for aa 1‑49. Over aa 47‑396, mouse USP2 shares 96% and 99% aa identity with human and rat USP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.