Recombinant Human USP14 Protein

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SDS-Page: USP14 Protein [NBP2-22921]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human USP14 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-494 of Human USP14

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPLYSVT VKWGKEKFEG VELNTDEPPM VFKAQLFALT GVQPARQKVM VKGGTLKDDD WGNIKIKNGM TLLMMGSADA LPEEPSAKTV FVEDMTEEQL ASAMELPCGL TNLGNTCYMN ATVQCIRSVP ELKDALKRYA GALRASGEMA SAQYITAALR DLFDSMDKTS SSIPPIILLQ FLHMAFPQFA EKGEQGQYLQ QDANECWIQM MRVLQQKLEA IEDDSVKETD SSSASAATPS KKKSLIDQFF GVEFETTMKC TESEEEEVTK GKENQLQLSC FINQEVKYLF TGLKLRLQEE ITKQSPTLQR NALYIKSSKI SRLPAYLTIQ MVRFFYKEKE SVNAKVLKDV KFPLMLDMYE LCTPELQEKM VSFRSKFKDL EDKKVNQQPN TSDKKSSPQK EVKYEPFSFA DDIGSNNCGY YDLQAVLTHQ GRSSSSGHYV SWVKRKQDEW IKFDDDKVSI VTPEDILRLS GGGDWHIAYV LLYGPRRVEI MEEESEQ

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
USP14
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human USP14 Protein

  • Deubiquitinating enzyme 14
  • EC 3.1.2.15
  • EC 3.4.19.12
  • TGT
  • TGTubiquitin carboxyl-terminal hydrolase 14
  • tRNA-guanine transglycosylase, 60-kD subunit
  • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
  • ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
  • Ubiquitin Thioesterase 14
  • Ubiquitin thiolesterase 14
  • ubiquitin-specific processing protease 14
  • Ubiquitin-specific-processing protease 14
  • Ubp6
  • USP14

Background

USP14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Recombinant human USP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol USP14
Entrez