USP14 Antibody

Western Blot: USP14 Antibody [H00009097-B01P] - Analysis of USP14 expression in rat brain.

Western Blot: USP14 Antibody [H00009097-B01P] - Analysis of USP14 expression in transfected 293T cell line by USP14 polyclonal antibody. Lane 1: USP14 transfected lysate(54.34 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

USP14 Antibody Summary

• Immunogen: USP14 (NP_005142.1, 1 a.a. - 494 a.a.) full-length human protein. MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ
• Specificity: USP14 - ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: USP14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP14 Antibody

• Deubiquitinating enzyme 14
• EC 3.1.2.15
• EC 3.4.19.12
• TGT
• TGTubiquitin carboxyl-terminal hydrolase 14
• tRNA-guanine transglycosylase, 60-kD subunit
• ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
• Ubiquitin Thioesterase 14
• Ubiquitin thiolesterase 14
• ubiquitin-specific processing protease 14
• Ubiquitin-specific-processing protease 14
• Ubp6
• USP14

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP14
• Entrez:
  • 9097(Human)
• Uniprot:
  • NP_005142.1(Human)