USP14 Antibody (JU30-49)

Western Blot: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of USP14 on K562 (1) and Hela (2) cell lysate using anti-USP14 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: USP14 Antibody (JU30-49) [NBP2-75700] - Staining USP14 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of paraffin-embedded rat epididymis tissue using anti-USP14 antibody. Counter stained with hematoxylin.

Flow Cytometry: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of Jurkat cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of USP14 on Zebrafish tissue lysates using anti-USP14 antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: USP14 Antibody (JU30-49) [NBP2-75700] - Staining USP14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: USP14 Antibody (JU30-49) [NBP2-75700] - Staining USP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of paraffin-embedded human colon cancer tissue using anti-USP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of paraffin-embedded human kidney tissue using anti-USP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP14 Antibody (JU30-49) [NBP2-75700] - Analysis of paraffin-embedded mouse prostate tissue using anti-USP14 antibody. Counter stained with hematoxylin.

Product Discontinued

USP14 Antibody (JU30-49) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human USP14 aa 351-494 / 494. (SwissProt: P54578 Human)
• Localization: Cytoplasm. Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: USP14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for USP14 Antibody (JU30-49)

• Deubiquitinating enzyme 14
• EC 3.1.2.15
• EC 3.4.19.12
• TGT
• TGTubiquitin carboxyl-terminal hydrolase 14
• tRNA-guanine transglycosylase, 60-kD subunit
• ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
• Ubiquitin Thioesterase 14
• Ubiquitin thiolesterase 14
• ubiquitin-specific processing protease 14
• Ubiquitin-specific-processing protease 14
• Ubp6
• USP14

Background

USP14 encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP14