USF1 Antibody (3F6)

Western Blot: USF1 Antibody (3F6) [H00007391-M01] - USF1 monoclonal antibody (M01), clone 3F6 Analysis of USF1 expression in K-562.

Western Blot: USF1 Antibody (3F6) [H00007391-M01] - Analysis of USF1 over-expressed 293 cell line, cotransfected with USF1 Validated Chimera RNAi ( Cat # H00007391-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with USF1 monoclonal antibody (M01), clone 3F6 (Cat # H00007391-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: USF1 Antibody (3F6) [H00007391-M01] - Analysis of USF1 expression in transfected 293T cell line by USF1 monoclonal antibody (M01), clone 3F6.Lane 1: USF1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.

ELISA: USF1 Antibody (3F6) [H00007391-M01] - Detection limit for recombinant GST tagged USF1 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

USF1 Antibody (3F6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: USF1 (AAH35505, 1 a.a. ~ 310 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
• Specificity: USF1 - upstream transcription factor 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USF1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • RNA Inhibition
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USF1 Antibody (3F6)

• BHLHB11
• bHLHb11FCHL1
• Class B basic helix-loop-helix protein 11
• FCHL
• HYPLIP1
• Major late transcription factor 1
• MLTF
• MLTFI
• UEF
• upstream stimulatory factor 1
• upstream transcription factor 1
• USF

Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USF1 Antibody (H00007391-M01)(1)

Publication using H00007391-M01

• Wong RH, Chang I, Hudak CS et al. A role of DNA-PK for the metabolic gene regulation in response to insulin. Cell. 2009-03-20 [PMID: 19303849]

Bioinformatics

• Gene Symbol: USF1
• Entrez:
  • 7391(Human)
• OMIM:
  • 191(Human)
• Uniprot:
  • AAH35505(Human)