UPF3A Antibody

Product Discontinued

UPF3A Antibody Summary

• Immunogen: Peptide with sequence C-KETDKQKKIAEKE corresponding to internal region according to NP_075387.1, NP_542418.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UPF3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 48 kDa band in lysates of HeLa and Kelly after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51 kDa band according to NP_542418.1 and the reported sizes of 65 to 70 kDa band from different sources. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for UPF3A Antibody

• HUPF3A
• Nonsense mRNA reducing factor 3A
• regulator of nonsense transcripts 3A
• RENT3AUp-frameshift suppressor 3 homolog A
• UPF3 regulator of nonsense transcripts homolog A (yeast)
• UPF3hUpf3

Background

UPF3A encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UPF3A Antibody (NBP1-45245)(1)

Publication using NBP1-45245

• Tarpey PS, Raymond FL, Nguyen LS et al. Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation. Nat Genet 2007-09-01 [PMID: 17704778]

Bioinformatics

• Gene Symbol: UPF3A
• Entrez:
  • 65110(Human)
• Uniprot:
  • NP_075387.1(Human)