UNC5H2/UNC5B Antibody (1A9)

Sandwich ELISA: UNC5H2/UNC5B Antibody (1A9) [H00219699-M01] - Detection limit for recombinant GST tagged UNC5B is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

UNC5H2/UNC5B Antibody (1A9) Summary

• Immunogen: UNC5B (NP_734465, 27 a.a. ~ 126 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDY
• Localization: Membrane; Single-pass type I membrane protein.
• Specificity: UNC5B - unc-5 homolog B (C. elegans)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UNC5B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UNC5H2/UNC5B Antibody (1A9)

• p53RDL1
• p53-regulated receptor for death and life protein 1
• Protein unc-5 homolog 2
• Protein unc-5 homolog B
• transmembrane receptor Unc5H2
• unc5 (C.elegans homolog) b
• unc-5 homolog 2
• unc-5 homolog B (C. elegans)
• UNC5B
• UNC5H2
• UNC5H2netrin receptor UNC5B

Background

UNC5B belongs to a family of netrin 1 receptors thought to mediate the chemorepulsive effect of netrin 1 on specific axons. Unc5B is required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC5H2/UNC5B Antibody (H00219699-M01)(1)

Publication using H00219699-M01

• Tang X, Jang SW, Okada M et al. Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Nat Cell Biol 2008-06-01 [PMID: 18469807] (WB, Human)

Bioinformatics

• Gene Symbol: UNC5B
• Entrez:
  • 219699(Human)
• OMIM:
  • 607870(Human)
• Uniprot:
  • NP_734465()