ULBP-4/RAET1E Antibody

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human ULBP-4/RAET1E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6285) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ULBP-4/RAET1E at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ULBP-4/RAET1E Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ULBP‑4/RAET1E
  Gly30-Asp225
  Accession # Q8TD07
• Specificity: Detects human ULBP‑4/RAET1E in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ULBP-1, rhULBP-2, and rhULBP-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAET1E
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-4/RAET1E Antibody

• bA350J20.7
• LETAL
• LETALRAE-1-like transcript 4
• Lymphocyte effector toxicity activation ligand
• MGC125309
• N2DL4
• N2DL-4
• NKG2D ligand 4
• NKG2DL4
• RAET1E
• RAET1E2
• retinoic acid early transcript 1ERL-4
• RL-4
• ULBP4
• ULBP-4
• ULBP4MGC125308

Background

ULBP-4 (UL16 binding protein 4; also NKG2D Ligand 4, Latel and RAET1E) is a 30-40 kDa glycoprotein member of the MHC Class I family of molecules. It has widespread tissue expression, and is particularly prominent on tumor cells. ULBP4 is a monomeric, non-antigen presenting MHC molecule that does not interact with beta ₂-microglobulin. It is a ligand for both TCR gamma δ and homodimeric NKG2D, the latter which appears on NK, CD8⁺ and  gamma δ T cells. NKG2D ligation induces cellular cytotoxicity and cytokine secretion. Mature human ULBP4 is a 234 amino acid (aa) type I transmembrane protein. It contains a 196 aa extracellular region (aa 30-225), and a short 15 aa cytoplasmic domain. Multiple splice variants exist. One shows a five aa substitution for aa 208-263, a second shows a deletion of aa 28-63, a third shows a two aa substitution for aa 208-263, while a fourth possesses an 18 aa substitution for Gly30. Soluble ULBP4 is described to be 35 kDa in size. There is no reported rodent counterpart to ULBP4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-4/RAET1E Antibody (AF6285)(2)

Publications using AF6285

• Y Han, W Xie, DG Song, DJ Powell Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells J Hematol Oncol, 2018-07-06;11(1):92. 2018-07-06 [PMID: 29980239] (CAR-T (Flow Cytometry), Human)
• Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J. Exp. Med., 2010-12-20;208(1):13-21. 2010-12-20 [PMID: 21173105] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: RAET1E
• Uniprot:
  • Q8TD07(Human)