UFM1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

UFM1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human UFM1
  Met1-Gly83
  Accession # P61960
• Specificity: Detects human UFM1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UFM1 Antibody [Alexa Fluor® 405]

• bA131P10.1
• BM-002
• C13orf20
• chromosome 13 open reading frame 20
• ubiquitin-fold modifier 1
• UFM1

Background

UFM1 ( Ubiquitin-fold modifier 1) is a 9.1 kDa ubiquitin-like protein, displaying a similar tertiary structure to ubiquitin. The UFM1 conjugation system is a novel Ubiquitin-like (Ubl) system whose physiological target(s) and biological functions remain largely undefined. To be activated, UFM1 is processed C-terminally by two specific proteases, UfSP1 and UfSP2.  After processing, UFM1 is activated via the E1 enzyme, UBA5, and then conjugated by the E2 enzyme, UFC1. UFL1 has been identified as the E3 enzyme. However, cellular functions associated with target proteins that are modified by UFM1 are still unknown. Genetic study has demonstrated that the Ufm1-activating enzyme Uba5 is indispensible for erythroid differentiation in mice, highlighting the importance of this novel system in animal development. It was found that the UFM1 system was transcriptionally up-regulated by disturbance of the ER homeostasis and inhibition of vesicle trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.