UFD1L Overexpression Lysate

Western Blot: UFD1L Overexpression Lysate (Adult Normal) [NBL1-17588] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UFD1L.

• Reactivity: Hu
• Applications: WB

UFD1L Overexpression Lysate Summary

• Description: Transient overexpression lysate of ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC213180 and based on accession number NM_001035247. The protein contains a C-MYC/DDK Tag.
• Gene: UFD1L

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 29.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UFD1L Overexpression Lysate

• ATP1C
• ATP1G1ATPase, Na+/K+ transporting, gamma 1 polypeptide
• FXYD domain containing ion transport regulator 2
• FXYD domain-containing ion transport regulator 2
• HOMG2
• hypomagnesemia 2, renal
• MGC12372
• Na(+)/K(+) ATPase subunit gamma
• Sodium pump gamma chain
• sodium/potassium-transporting ATPase subunit gamma
• Sodium-potassium-ATPase, gamma polypeptide

Background

The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, that is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, compared to variant 1, resulting in a shorter isoform (B), compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: UFD1L