Uev1a/UBE2V1 Antibody

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Western Blot: Uev1a/UBE2V1 Antibody [NB100-56397] - Analysis of UBE2V1a in Jurkat cell lysate using this antibody at 1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Uev1a/UBE2V1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 31-49 of human UBE2V1a. Genbank accession no. NP_954595.1.
Specificity
A protein band of approximate mloecular weght of 20 kD can be seen in Jurkat, RAW and HeLa cell lysates. This may also recognize isoform b of UBE2V1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2V1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
Positive Control
Uev1a/UBE2V1 Lysate (NBL1-17547)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Uev1a/UBE2V1 Antibody

  • CIR1
  • CROC1
  • CROC1TRAF6-regulated IKK activator 1 beta Uev1A
  • CROC-1UBE2V
  • UBE2V1
  • ubiquitin-conjugating enzyme E2 variant 1
  • Uev1a
  • UEV-1CIR1
  • UEV1DNA-binding protein

Background

The E2 enzyme, Ubc13, and the E2 enzyme variants, Uevs, form stable, high affinity complexes for the assembly of Lys63-linked ubiquitin chains. This complex has been shown to activate NF-kB signaling pathway by linking TRAF6 to IKKs. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results suggest a new regulatory function for ubiquitin, in which IKK is activated through the assembly of Lys63-linked polyubiquitin chains. The UEV proteins are also involved in the control of differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol UBE2V1
Entrez