UBTF Antibody

Product Discontinued

UBTF Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human UBF1. The immunogen region is: Internal.
• Specificity: UBF1 Antibody detects endogenous levels of total UBF1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBTF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBTF Antibody

• 90-KDa Nucleolus Organizer Region Autoantigen
• Autoantigen NOR-90
• NOR-90
• nucleolar transcription factor 1
• UBF
• UBF1
• UBF-1
• UBF2
• UBFUBF-1
• upstream binding transcription factor, RNA polymerase I
• Upstream-binding factor 1

Background

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBTF
• Uniprot:
  • P17480(Human)