UBTF Antibody

Product Discontinued

UBTF Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human UBF-1.
• Specificity: UBF-1 (E536) detects endogenous levels of UBF-1 (E536) protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBTF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of UBF-1 (E536) pAb in extracts from HeLa cells.
  Immunohistochemistry (IHC) analyzes of UBTF (E536) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat,.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBTF Antibody

• 90-KDa Nucleolus Organizer Region Autoantigen
• Autoantigen NOR-90
• NOR-90
• nucleolar transcription factor 1
• UBF
• UBF1
• UBF-1
• UBF2
• UBFUBF-1
• upstream binding transcription factor, RNA polymerase I
• Upstream-binding factor 1

Background

The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the 70 kDa and 86 kDa Ku antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBTF
• Entrez:
  • 7343(Human)
  • 21429(Mouse)
  • 25574(Rat)