UBE2S Knockout 293T Cell Lysate

Product Discontinued

UBE2S Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 2
• Gene: UBE2S

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for UBE2S Knockout 293T Cell Lysate

• E2-24K
• E2-EPF
• E2-EPF5
• E2-EPF52
• E2-EPFEC 6.3.2.19
• E2EPFEPF5
• EPF5
• UBE2S
• Ubiquitin carrier protein S
• ubiquitin-conjugating enzyme E2 S
• ubiquitin-conjugating enzyme E2-24 kD
• Ubiquitin-conjugating enzyme E2-24 kDa
• Ubiquitin-conjugating enzyme E2-EPF5
• ubiquitin-conjugating enzyme E2S
• Ubiquitin-protein ligase S

Background

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2S