UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-UBE2R1/CDC34-2).

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-UBE2R1/CDC34-2).

Protein Array: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-CDC34-2).

• Reactivity: Hu
• Applications: IHC, MA
• Clone: CPTC-CDC34-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UBE2R1/CDC34 Antibody (CPTC-CDC34-2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08839)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human full-length UBE2R1/CDC34 protein (Uniprot: P49427)
• Localization: Nucleus, Cytoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC34
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

• Cdc34
• cell division cycle 34 homolog (S. cerevisiae)
• cell division cycle 34
• E2-CDC34
• EC 6.3.2.19
• UBC3
• UBE2R1
• UBE2R1UBCH3
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 R1
• Ubiquitin-conjugating enzyme E2-32 kDa complementing
• Ubiquitin-conjugating enzyme E2-CDC34
• Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC34
• Uniprot:
  • P49427(Human)