UBE2N/Ubc13 Antibody (S05-5B0)

Western Blot: UBE2N/Ubc13 Antibody (S05-5B0) [NBP3-20010] - Western blot detection of UBE2N/Ubc13 in HL-60, C6 using NBP3-20010 (1:1000 diluted)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-5B0
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

UBE2N/Ubc13 Antibody (S05-5B0) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human UBE2N/Ubc13 (Uniprot # P61088)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBE2N
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for UBE2N/Ubc13 Antibody (S05-5B0)

• bendless-like ubiquitin conjugating enzyme
• Bendless-like ubiquitin-conjugating enzyme
• BLU
• EC 6.3.2.19
• MGC131857
• MGC8489
• UBC13
• UbcH13
• UbcH-ben
• UBE2N
• Ubiquitin carrier protein N
• ubiquitin-conjugating enzyme E2 N
• ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
• ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Ubiquitin-protein ligase N
• yeast UBC13 homolog

Background

UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 which then catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-free DNA repair. UBC13 also contributes to the survival of cells after DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2N