UBE2I/Ubc9 Antibody

Images

 
Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).
Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human tonsil shows moderate to strong nuclear positivity in non - germinal center cells.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

UBE2I/Ubc9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQ
Specificity
Specificity of human, mouse, rat UBE2I/Ubc9 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2I
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Reviewed Applications
Read 1 Review rated 4
using
NBP1-86887 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBE2I/Ubc9 Antibody

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for UBE2I/Ubc9 Antibody (NBP1-86887) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NBP1-86887:
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Images Ratings Applications Species Date Details
Western Blot UBE2I/Ubc9 NBP1-86887
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reviewed by:
Anonymous
WB Human and Mouse 06/29/2017
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Summary

ApplicationWestern Blot
Sample Tested293FT cell lysate,nih3t3 cell lysate
SpeciesHuman and Mouse
Lotr07577

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Anonymous
06/29/2017
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol UBE2I