| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86887-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93799-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00096-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20104-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86887-img0006.jpg "Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0010.jpg)
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0007.jpg)
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human tonsil shows moderate to strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0008.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-86887_rabbit-polyclonal-ube2i-ubc9-antibody-30420251391778.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NB300-812-img0007.jpg "Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NB300-812-img0008.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NB300-812-img0004.jpg)
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NB300-812-img0006.jpg)
![Western Blot: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Western blot analysis of extracts of various cell lines, using UBE2I/Ubc9 Rabbit mAb (NBP3-16400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-6Z9V6-Western-Blot-NBP3-16400-img0004.jpg "Western Blot: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Western blot analysis of extracts of various cell lines, using UBE2I/Ubc9 Rabbit mAb (NBP3-16400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Western blot analysis of extracts of HeLa cells, using UBE2I/Ubc9 Rabbit mAb (NBP3-16400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-6Z9V6-Western-Blot-NBP3-16400-img0005.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded human thyroid cancer tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-7220251245326.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded rat colon tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-7220251245359.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded mouse testis tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-72202512435350.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded rat brain tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-7220251245374.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded mouse brain tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-72202512453710.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded mouse lung tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-7220251245366.jpg)
![Immunohistochemistry: UBE2I/Ubc9 Antibody (6Z9V6) [NBP3-16400] - Immunohistochemistry analysis of UBE2I/Ubc9 in paraffin-embedded mouse spleen tissue using UBE2I/Ubc9 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16400_rabbit-ube2i-ubc9-mab-6z9v6-7220251245332.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NBP2-93799] - Western blot analysis of extracts of various cell lines, using (NBP2-93799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/UBE2I-Ubc9 Antibody-Western Blot-NBP2-93799-img0002.jpg "Western Blot: UBE2I/Ubc9 Antibody [NBP2-93799] - Western blot analysis of extracts of various cell lines, using (NBP2-93799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP2-93799] - Immunohistochemistry of paraffin-embedded rat lung using UBE2I/Ubc9 Rabbit pAb (NBP2-93799) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP2-93799-img0001.jpg)
![Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00096 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00096, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa](http://images.novusbio.com/fullsize/nbp3-00096_rabbit-polyclonal-ube2i-ubc9-antibody-235202511545648.png "Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00096 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00096, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa")
![Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - NBP3-00096 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa](http://images.novusbio.com/fullsize/nbp3-00096_rabbit-polyclonal-ube2i-ubc9-antibody-235202516335011.png)
![Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00096 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp3-00096_rabbit-polyclonal-ube2i-ubc9-antibody-235202516144328.png)
![Flow Cytometry: UBE2I/Ubc9 Antibody (SAIC-36A-9) - Azide and BSA Free [NBP3-20104] - Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-36A-9 (NBP3-20104, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20104_rabbit-ube2i-ubc9-mab-saic-36a-9-azide-and-bsa-free-2742023924280.png "Flow Cytometry: UBE2I/Ubc9 Antibody (SAIC-36A-9) - Azide and BSA Free [NBP3-20104] - Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-36A-9 (NBP3-20104, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody (SAIC-36A-9) - Azide and BSA Free [NBP3-20104] - Immunofluorescence analysis of paraformaldehyde fixed Jurak cells on Shi-fix(TM) cover-slips stained with the chimeric rabbit IgG version of SAIC-36A-9 (NBP3-20104) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20104, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20104_rabbit-ube2i-ubc9-mab-saic-36a-9-azide-and-bsa-free-2742023924286.png)
![Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-10070] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.](http://images.novusbio.com/fullsize/UBE2I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10070-img0001.jpg "Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-10070] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.")
![Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-04755] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.](http://images.novusbio.com/fullsize/UBE2I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04755-img0001.jpg "Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-04755] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.")
![Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBL1-17533] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.](http://images.novusbio.com/fullsize/UBE2I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17533-img0002.jpg "Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBL1-17533] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.")
![Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-10069] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.](http://images.novusbio.com/fullsize/UBE2I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10069-img0001.jpg "Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBP2-10069] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.")
![Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBL1-17532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.](http://images.novusbio.com/fullsize/UBE2I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17532-img0002.jpg "Western Blot: UBE2I Overexpression Lysate (Adult Normal) [NBL1-17532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE2I.")