Recombinant Human UBE2F/NCE2 Protein

Product Discontinued

Recombinant Human UBE2F/NCE2 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-185 of Human UBE2F/NCE2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2F
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 1 mM DTT, 200 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UBE2F/NCE2 Protein

• EC 6.3.2
• EC 6.3.2.-
• MGC18120
• NCE2
• NCE2Ubiquitin-conjugating enzyme E2 F
• NEDD8 carrier protein UBE2F
• NEDD8 conjugating enzyme
• NEDD8 protein ligase UBE2F
• NEDD8-conjugating enzyme 2
• NEDD8-conjugating enzyme UBE2F
• UBE2F
• ubiquitin-conjugating enzyme E2F (putative)

Background

UBE2F, also Known as Ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2F