Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free

Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 147 amino acids corresponding to UbcH5c/UBE2D3 and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

  Uniprot ID: P61077

  Amino Acid Sequence: MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2D3
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of UbcH5c/UBE2D3, His as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein

• E2(17)KB 3
• E2(17)KB3
• EC 6.3.2.19
• MGC43926
• MGC5416
• UBC4/5
• UBC5C
• UbcH5c
• UBE2D3
• Ubiquitin carrier protein D3
• ubiquitin-conjugating enzyme E2 D3
• Ubiquitin-conjugating enzyme E2(17)KB 3
• Ubiquitin-conjugating enzyme E2-17 kDa 3
• ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
• ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D3

Background

Ubiquitin Conjugating Enzyme E2 D3 (UBE2D3)/UBC5C enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, I kappa B alpha, p105) and abnormal proteins. UBE2D3 has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D3
• Uniprot:
  • P61077(Human)