UbcH5c/UBE2D3 Antibody (4C1-1E3) - Azide and BSA Free

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - UBE2D3 monoclonal antibody (M01), clone 4C1-1E3 Analysis of UBE2D3 expression in Jurkat.

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - Antibody Reactive Against Recombinant Protein. Western Blot detection against Immunogen (42.28 KDa) .

Western Blot: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - Analysis of UBE2D3 expression in transfected 293T cell line by UBE2D3 monoclonal antibody (M01), clone 4C1-1E3.Lane 1: UBE2D3 transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: UbcH5c/UBE2D3 Antibody (4C1-1E3) [H00007323-M01] - Detection limit for recombinant GST tagged UBE2D3 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4C1-1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UbcH5c/UBE2D3 Antibody (4C1-1E3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: UBE2D3 (AAH03395, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM
• Specificity: UBE2D3 - ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2D3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UbcH5c/UBE2D3 Antibody (4C1-1E3) - Azide and BSA Free

• E2(17)KB 3
• E2(17)KB3
• EC 6.3.2.19
• MGC43926
• MGC5416
• UBC4/5
• UBC5C
• UbcH5c
• UBE2D3
• Ubiquitin carrier protein D3
• ubiquitin-conjugating enzyme E2 D3
• Ubiquitin-conjugating enzyme E2(17)KB 3
• Ubiquitin-conjugating enzyme E2-17 kDa 3
• ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
• ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D3
• Entrez:
  • 7323(Human)
• OMIM:
  • 602963(Human)
• Uniprot:
  • AAH03395(Human)