Recombinant Human UbcH10/UBE2C His (N-Term) Protein

SDS-Page: Human UbcH10/UBE2C His (N-Term) Protein [NBP2-35090]

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free

Recombinant Human UbcH10/UBE2C His (N-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 179 amino acids corresponding to UbcH10/UBE2C and 12 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

  Amino Acid Sequence: MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2C
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of UbcH10/UBE2C as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human UbcH10/UBE2C His (N-Term) Protein

• cyclin-selective ubiquitin carrier protein
• dJ447F3.2
• EC 6.3.2.19
• UbcH10
• UBCH10mitotic-specific ubiquitin-conjugating enzyme
• UBE2C
• Ubiquitin carrier protein C
• ubiquitin carrier protein E2-C
• ubiquitin-conjugating enzyme E2 C
• ubiquitin-conjugating enzyme E2C
• Ubiquitin-protein ligase C

Background

Human Ubquitin Conjugating Enzyme E2 C (UBE2C)/UBCH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2C