UbcH10/UBE2C Antibody

Images

 
Western Blot: UbcH10/UBE2C Antibody [NB100-1084] - (2ug/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Flow Cytometry: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more
Immunocytochemistry/ Immunofluorescence: UbcH10/UBE2C Antibody [NB100-1084] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UbcH10/UBE2C Antibody Summary

Immunogen
Peptide with sequence C-QETYSKQVTSQEP corresponding to C-Terminus according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
Specificity
This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBE2C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
Flow Cytometry: Flow cytometric analysis of HeLa cells.
Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
Control
UbcH10/UBE2C Overexpression Lysate
UbcH10/UBE2C Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-1084.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH10/UBE2C Antibody

  • cyclin-selective ubiquitin carrier protein
  • dJ447F3.2
  • EC 6.3.2.19
  • UbcH10
  • UBCH10mitotic-specific ubiquitin-conjugating enzyme
  • UBE2C
  • Ubiquitin carrier protein C
  • ubiquitin carrier protein E2-C
  • ubiquitin-conjugating enzyme E2 C
  • ubiquitin-conjugating enzyme E2C
  • Ubiquitin-protein ligase C

Background

Ubiquitin-conjugating enzyme E2 C (UBE2C) catalyzes the covalent attachment of ubiquitin to other proteins. Required for the destruction of mitotic cyclins UBE2C carries out the ubiquitin conjugation second step, ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UbcH10/UBE2C Antibody (NB100-1084)(1)

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol UBE2C
Entrez
Uniprot