UAP1 Antibody - BSA Free

Western Blot: UAP1 Antibody [NBP1-32823] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:3000

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-32823] - UAP1 Antibody detects UAP1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UAP1 protein stained by UAP1 Antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-32823] - Paraffin-embedded ES-2 xenograft, using antibody at 1:500 dilution.

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-32823] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UAP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UAP1 Antibody - BSA Free (NBP1-32823) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UAP1 Antibody - BSA Free

• AGX
• AGX1
• AGX2
• Antigen X
• EC 2.7.7
• EC 2.7.7.23
• EC 2.7.7.27
• SPAG2
• sperm associated antigen 2
• Sperm-associated antigen 2
• UDP-N-acetylhexosamine pyrophosphorylase
• UDP-N-acteylglucosamine pyrophosphorylase 1

Background

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UAP1