Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tyrosylprotein Sulfotransferase 2/TPST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6236) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tyrosylprotein Sulfotransferase 2/TPST2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Tyrosylprotein Sulfotransferase 2/TPST2 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recominant human Tyrosylprotein Sulfotransferase 2/TPST2
  Gln26-Ser377 (predicted)
  Accession # O60704
• Specificity: Detects human Tyrosylprotein Sulfotransferase 2/TPST2 in direct ELISAs and Western blots. In direct ELISAs, less than 7% cross-reactivity with recombinant human TPST1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TPST2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

• EC 2.8.2.20
• TPST2
• TPST-2
• tyrosylprotein phosphotransferase 2
• Tyrosylprotein Sulfotransferase 2
• tyrosylprotein sulfotransferase 2protein-tyrosine sulfotransferase 2
• tyrosylprotein sulfotransferase-2

Background

Human Tyrosylprotein Sulfotransferase 2 (TPST2) is a 42 kDa (predicted) member of a sulfotransferase family. Two human tyrosylprotein sulfotransferases (TPSTs), TPST1 and TPST2 mediate tyrosine O-sulfation of more than 60 different proteins in the Golgi, transferring sulfate from 3'-phosphoadenosine 5'‑phosphosulfate to tyrosine residues contained in polypeptides with acidic motifs to form a tyrosine O-sulfate ester. TSPT1 and TSPT2 are widely expressed, they show similar tissue distribution and overlapping substrate specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Tyrosylprotein Sulfotransferase 2/TPST2 Antibody (AF6236). (Showing 1 - 1 of 1 FAQ).

1. What is the lower limit of detection for TPST2 in Western blot?
   • We generally do not test the limits of detection for our antibodies as this time. I apologize for the inconvenience.

Bioinformatics

• Gene Symbol: TPST2