TXNRD2 Antibody - BSA Free

Western Blot: TXNRD2 Antibody [NBP2-94256] - Analysis of extracts of various cell lines, using TXNRD2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Immunohistochemistry-Paraffin: TXNRD2 Antibody [NBP2-94256] - Paraffin-embedded human liver damage using TXNRD2 .

Immunohistochemistry-Paraffin: TXNRD2 Antibody [NBP2-94256] - Paraffin-embedded human liver cancer using TXNRD2 .

Immunocytochemistry/ Immunofluorescence: TXNRD2 Antibody - BSA Free [TXNRD2] - Immunofluorescence analysis of U2OS cells using TXNRD2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: TXNRD2 Antibody - BSA Free [TXNRD2] - Immunofluorescence analysis of L929 cells using TXNRD2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TXNRD2 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2). AALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TXNRD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TXNRD2 Antibody - BSA Free

• EC 1.8.1
• EC 1.8.1.9
• KIAA1652
• Selenoprotein Z
• SelZ
• Thioredoxin Reductase 2
• thioredoxin reductase 3
• thioredoxin reductase beta
• Thioredoxin reductase TR3
• TR
• TR3
• TR-beta
• TRXR2
• TRXR2thioredoxin reductase 2, mitochondrial
• TXNRD2

Background

Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins. This gene partially overlaps the COMT gene on chromosome 22. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXNRD2