Tubulin alpha-8 Antibody (2D6)

Western Blot: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Western blot analysis using TUBA8 mouse mAb against rat heart (1) tissue lysate.

Immunocytochemistry/Immunofluorescence: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Immunofluorescence analysis of Hela cells using TUBA8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Analysis of liver cancer tissues using TUBA8 mouse mAb with DAB staining.

Flow Cytometry: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Flow cytometric analysis of NIH/3T3 cells using TUBA8 mouse mAb (green) and negative control (red).

Western Blot: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Western blot analysis using TUBA8 mAb against human TUBA8 (AA: 294-449) recombinant protein. (Expected MW is 50 kDa)

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Analysis of medulla oblongata tissues using TUBA8 mouse mAb with DAB staining.

ELISA: Tubulin alpha-8 Antibody (2D6) [NBP2-37535] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Tubulin alpha-8 Antibody (2D6) Summary

• Immunogen: Purified recombinant fragment of human Tubulin alpha-8 expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Tubulin alpha-8 Antibody (2D6)

• Alpha-tubulin 8
• TUBA8
• TUBAL2
• TUBAL2Tubulin alpha chain-like 2
• tubulin alpha-8 chain
• Tubulin alpha-8
• tubulin, alpha 8

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tubulin alpha-8 Antibody (NBP2-37535)(1)

Publication using NBP2-37535

• Hausrat TJ, Radwitz J, Lombino FL et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development Dev Neurobiol 2020-04-15 [PMID: 32293117] (WB, Human)

Bioinformatics

• Gene Symbol: TUBA8