Recombinant Human TSTA3 Protein

Images

 
Western Blot: TSTA3 Protein [NBP1-45309] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human TSTA3 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-321 of Human TSTA3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TSTA3
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 50mM NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human TSTA3 Protein

  • EC 1.1.1.271
  • FX
  • GDP-4-keto-6-deoxy-D-mannose epimerase-reductase
  • GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
  • GDP-L-fucose synthase
  • P35B
  • Protein FX
  • Red cell NADP(H)-binding protein
  • SDR4E13-5 epimerase/4-reductase
  • short chain dehydrogenase/reductase family 4E, member 1
  • Short-chain dehydrogenase/reductase family 4E member 1
  • tissue specific transplantation antigen 3
  • tissue specific transplantation antigen P35B
  • Tissue-specific transplantation antigen-3

Background

TSTA3 is a NADP(H)-binding protein. It catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. Recombinant human TSTA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TSTA3