TSPAN33 Antibody (545422) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated overnight with Recombinant Human IL‑4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) were stained with Mouse Anti-Human TSPAN33 Monoclonal Antibody (Catalog # MAB8405) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 545422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSPAN33 Antibody (545422) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TSPAN33
  Met1-Tyr283
  Accession # NP_848657
• Specificity: Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSPAN33
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN33 Antibody (545422) [Unconjugated]

• hPen
• PEN
• Penumbra
• proerythroblast new membrane
• tetraspanin 33
• tetraspanin-33
• TSPAN33
• tspan-33

Background

TSPAN33 is a member of the tetraspanin family and is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. TSPAN33 is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma. TSPAN33 is also expressed in some autoimmune diseases where B cells participate in the pathology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN33 Antibody (MAB8405)(1)

Publication using MAB8405

• A Sohni, K Tan, HW Song, D Burow, DG de Rooij, L Laurent, TC Hsieh, R Rabah, SS Hammoud, E Vicini, MF Wilkinson The Neonatal and Adult Human Testis Defined at the Single-Cell Level Cell Rep, 2019-02-05;26(6):1501-1517.e4. 2019-02-05 [PMID: 30726734] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TSPAN33
• Uniprot:
  • NP_848657()