TSLPR/CRLF2 Antibody (179134) [Unconjugated]

Western blot shows lysates of NS0 mouse myeloma cell line mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human TSLP R Monoclonal Antibody (Catalog # MAB9811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NS0 cells transfected with hTSLPR (filled histogram) vs Irrelevant NS0 transfectant cells (open histogram) were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB9811) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 179134
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TSLPR/CRLF2 Antibody (179134) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TSLP R
  Gly25-Lys231
  Accession # Q9HC73
• Specificity: Detects human TSLP R in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLPR/CRLF2 Antibody (179134) [Unconjugated]

• CRL2
• CRL2cytokine receptor CRL2 precusor
• CRLF2
• CRLF2Y
• CRLM-2
• Cytokine receptor-like 2
• cytokine receptor-like factor 2
• ILXR
• IL-XR
• P2RY8/CRLF2 fusion
• PCOR1
• Thymic stromal lymphopoietin protein receptor
• thymic stromal lymphopoietin receptor
• thymic stromal-derived lymphopoietin receptor
• TSLP R
• TSLP receptor
• TSLPR

Background

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c⁺ dendritic cells (5).

1. Fujio, K. et al. (2000) Blood 95:2204.
2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9HC73()