Recombinant Human TSHR Protein

Product Discontinued

Recombinant Human TSHR Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-764 of Human TSHR full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence:MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHEMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL

• Preparation Method: in vitro wheat germ expression system with proprietary liposome technology
• Protein/Peptide Type: Recombinant Protein
• Gene: TSHR

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Western Blot
• Application Notes: Use in Western Blot reported in scientific literature (PMID: 23630351). In Vitro Wheat Germ System + Liposome Technology offers significant advantages over commonly used protein expression platforms:
  
  Higher Yield
  Greater Stability
  High Expression Success Rate
  Native Protein Conformation
  
  This protein has not yet been tested for use in functional studies and/or compound screening.
  All experiments requiring biological activity of the protein will not be backed by the Novus Guarantee or be eligible for the Innovator's Reward™ and must be undertaken at the customer's own risk.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Preservative: No Preservative

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human TSHR Protein

• CHNG1
• LGR3
• LGR3hTSHR-I
• MGC75129
• seven transmembrane helix receptor
• thyroid stimulating hormone receptor
• Thyroid-stimulating hormone receptor
• thyrotropin receptor
• thyrotropin receptor-I, hTSHR-I
• TSH R
• TSHR
• TSH-R

Background

Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for TSHR Recombinant Protein (H00007253-G01)(3)

Publications using H00007253-G01

• Kumata K, Nagata K, Matsushita M et al. Thyrotropin Receptor Antibody (TRAb)-IgM Levels Are Markedly Higher Than TRAb-IgG Levels in Graves' Disease Patients and Controls, and TRAb-IgM Production Is Related to Epstein-Barr Virus Reactivation. Viral Immunol 2016-08-16 [PMID: 27529807]
• Nagata K, Nakayama Y, Higaki K et al. Reactivation of persistent Epstein-Barr virus (EBV) causes secretion of thyrotropin receptor antibodies (TRAbs) in EBV-infected B lymphocytes with TRAbs on their surface. Autoimmunity 2015-03-11 [PMID: 25759125]
• Hargreaves CE, Grasso M, Hampe CS et al. Yersinia enterocolitica Provides the Link between Thyroid-Stimulating Antibodies and Their Germline Counterparts in Graves' Disease. J Immunol 2013-06-01 [PMID: 23630351] (WB)

Bioinformatics

• Gene Symbol: TSHR