TSHR Antibody (49)

Immunocytochemistry/Immunofluorescence: TSH R Antibody (49) [NB120-5492] - Analysis of Thyrotropin Receptor in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyrotropin Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Thyrotropin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Product Discontinued

TSHR Antibody (49) Summary

• Immunogen: Purified extracellular domain of recombinant human thyrotropin receptor.
• Epitope: This antibody recognizes an epitope between amino acid residues 277-296 of human TSH receptor.
• Localization: Cell Membrane
• Specificity: NB120-5492 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSHR
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Western Blot 1:500
• Application Notes: WB: Detects an approx. 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSHR respectively, in transfected Sf9 cell extract. ELISA: Used in TSH competitive inhibition studies.
• Control:

  TSHR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TSHR Antibody (49)

• CHNG1
• LGR3
• LGR3hTSHR-I
• MGC75129
• seven transmembrane helix receptor
• thyroid stimulating hormone receptor
• Thyroid-stimulating hormone receptor
• thyrotropin receptor
• thyrotropin receptor-I, hTSHR-I
• TSH R
• TSHR
• TSH-R

Background

The thyroid stimulating hormone (thyrotropin) receptor (TSHR) is expressed on the surface of thyrocyte cell membranes and is crucial for thyroid gland growth and function. TSHR’s are targets for the pathogenesis of many autoimmune diseases including Graves’ disease and primary myxedema. This 7-transmembrane-domain protein contains 5 potential extracellular N-linked glycosylation sites. Antibodies to TSHR can affect receptor function in a number of different ways. TSHR antibodies which can inhibit TSH binding to the receptor are called TSH binding inhibitor immunoglobulins or TBIIs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSHR Antibody (NB120-5492)(1)

Publication using NB120-5492

• Jorgensen MM, Baek R, Sloth J et al. Treatment with intravenous immunoglobulin increases the level of small EVs in plasma of pregnant women with recurrent pregnancy loss J. Reprod. Immunol. 2020-04-09 [PMID: 32330775] (Human)

Bioinformatics

• Gene Symbol: TSHR
• Entrez:
  • 7253(Human)