TSC2 [p Thr1462] Antibody

Product Discontinued

TSC2 [p Thr1462] Antibody Summary

• Immunogen: synthesized phosphopeptide derived from human Tsc2 around the phosphorylation site of threonine 1462(G-Y-TP-I-S).
• Modification: p Thr1462
• Localization: Cytoplasm, Membrane; Peripheral membrane protein. Note:At steady state found in association with membranes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TSC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TSC2 [p Thr1462] Antibody

• LAM
• LAMFLJ43106
• TSC2
• TSC4
• Tuberin
• Tuberous sclerosis 2 protein
• tuberous sclerosis 2

Background

Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder that is caused by mutation in either the TSC1 (hamartin) or TSC2 (tuberin) gene. TSC1 and TSC2 proteins form a physical and functional heterodimer in vivo that inhibits ribosomal protein S6 kinase 1 (S6K1) and activates eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) by inhibition of the mammalian target of rapamycin (mTOR). TSC2 belongs to small GTPase protein of the Ras superfamily, contain a Rap-GAP domain. Predicted molecular weight 201 kD. TSC2 is directly phosphorylated and inhibited by Akt that is involved in stimulating cell growth and is activated by growth stimulating signals, such as insulin. Thr1462 of TSC2 is Akt-regulated phosphorylation site. The Poly6358 antibody recognizes human phospho-TSC2 (Thr1462) and has been shown to be useful for immunoflorence staining. This antibody does not detect TSC2 phosphorylated at other sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSC2
• Entrez:
  • 7249(Human)
• Uniprot:
  • P49815()