Recombinant Human Troponin T Type 2 (cardiac) Protein

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SDS-Page: Recombinant Human Troponin T Type 2 (cardiac) Protein [NBC1-28765]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human Troponin T Type 2 (cardiac) Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-285 of Human Troponin T Type 2 (cardiac)

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNNT2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
36.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBC1-28765 in the following application:

Publications
Read Publication using NBC1-28765.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.5M NaCl, 0.1mM PMSF, 1mM DTT, 100mM imidazole
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Troponin T Type 2 (cardiac) Protein

  • cardiac
  • CMD1D
  • CMPD2
  • dilated 1D (autosomal dominant)
  • hypertrophic 2
  • MGC3889
  • RCM3
  • TnTc
  • troponin T type 2 (cardiac)
  • troponin T, cardiac muscle

Background

Troponin T Type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Troponin T Type 2 (cardiac) Recombinant Protein (NBC1-28765)(1)

We have publications tested in 1 application: ELISA.


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(1)
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Review for Troponin T Type 2 (cardiac) Recombinant Protein (NBC1-28765) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Reviews using NBC1-28765:
Filter by Applications
ELISA
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 12/23/2014
View

Summary

ApplicationELISA
Sample TestedSee PMID 23906320
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23906320

Details

Detection NotesSee PMID 23906320

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23906320.

FAQs for Troponin T Type 2 (cardiac) Recombinant Protein (NBC1-28765). (Showing 1 - 1 of 1 FAQ).

  1. A customer is looking for an antigen Cardiac Troponin T for her ELISA, but it must not have a His-tag and no mercaptoethanol in the buffer. Would this be true for the antigen NBC1-28765? In addition, the customer wants to be sure that it really is a Cardiac Troponin T, because on your website some different information are given, like TNNT1 which is slow skeletal muscle.
    • I have carried out a search and I cannot find a suitable protein that matches your customers request. Product NBC1-28765 is not a Cardiac Troponin T Protein. It is slow skeletal muscle. I am very sorry that we do not have what your customer is looking for!

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
12/23/2014
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol TNNT2