Troponin T Type 2 (cardiac) Antibody (7G7)

Product Discontinued

Troponin T Type 2 (cardiac) Antibody (7G7) Summary

• Immunogen: Full length protein (Human).
• Localization: Cytoplasmic
• Specificity: Does not cross-react with skeletal muscle troponin T, cTnT or TnC.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
• Application Notes: Troponin T immunoassay. It is possible to use following pairs of antibodies for sandwich immunoassay (coating - conjugate): NB120-10223 (coating)-NBP1-79059 (Conjugate)NB120-10223 (Coating)-NB200-412 (Conjugate)NB120-10223 (Coating)-NB120-10216 (Conjugate)This antibody can be used as a capture for BIAcore.

Reactivity Notes

Cross-reacts with Human and Cow.Does not cross-react with Mouse, Rat, Cat, Dog, Fish, Goat, Pig or Rabbit. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (7G7)

• cardiac
• CMD1D
• CMPD2
• dilated 1D (autosomal dominant)
• hypertrophic 2
• MGC3889
• RCM3
• TnTc
• troponin T type 2 (cardiac)
• troponin T, cardiac muscle

Background

Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cardiac Troponin T Antibody (NB120-10223). (Showing 1 - 1 of 1 FAQ).

1. Regarding the epitope aa 60-70 for Cat# NB120-10233 (anti-Troponin T, cardiac), may I know if this number is based on the canonical cTNT protein sequence which is isoform 1, or on isoform 6 (the adult isoform) of cTNT?
   • The peptide immunogen used to generate this antibody comes from isoform 6 of cTNT. Based on a BLAST of the immunogen it does look like this antibody should recognize all isoforms.

Bioinformatics

• Gene Symbol: TNNT2
• Entrez:
  • 7139(Human)
• Uniprot:
  • P45379()