Troponin T Type 2 (cardiac) Antibody (3-G6)

Western Blot: Troponin T Type 2 (cardiac) Antibody (3-G6) [NBP2-75430] - Analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (3-G6) [NBP2-75430] - Staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (3-G6) [NBP2-75430] - Analysis of mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (3-G6) [NBP2-75430] - Staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Troponin T Type 2 (cardiac) Antibody (3-G6) Summary

• Immunogen: Synthetic peptide within huamn Troponin T Type 2 (cardiac) aa 45-89/298. (SwissProt: P45379 Human; SwissProt: P50752 Mouse)
• Localization: Cytoskeleton, Cytosol.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNT2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (3-G6)

• cardiac
• CMD1D
• CMPD2
• dilated 1D (autosomal dominant)
• hypertrophic 2
• MGC3889
• RCM3
• TnTc
• troponin T type 2 (cardiac)
• troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNT2